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Addgene

MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP
(Plasmid #75121)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-transgene-PGK-Puro IRES-GFP
  • Backbone size w/o insert (bp) 7656
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRD9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1818
  • Mutation
    Bromodomain replaced with the bromodomain of BRG1
  • Entrez Gene
    BRD9 (a.k.a. LAVS3040, PRO9856, SMARCI2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer MSCV5
  • 3′ sequencing primer MSCV3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP was a gift from Christopher Vakoc (Addgene plasmid # 75121 ; http://n2t.net/addgene:75121 ; RRID:Addgene_75121)

  • For your References section:

    Sensitivity and engineered resistance of myeloid leukemia cells to BRD9 inhibition. Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G, McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, Suzuki Y, Koegl M, Vakoc CR. Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115. 10.1038/nchembio.2115 PubMed 27376689
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