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Addgene

pCIneo RYR-1
(Plasmid #75113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5462
  • Total vector size (bp) 20594
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ryanodine receptor 1
  • Alt name
    RYR1
  • Species
    Rabbit
  • Insert Size (bp)
    15132
  • GenBank ID
    NM_001101718
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Paul D Allen, UC Davis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo RYR-1 was a gift from Paul Allen & Robert Dirksen (Addgene plasmid # 75113 ; http://n2t.net/addgene:75113 ; RRID:Addgene_75113)

  • For your References section:

    Two regions of the ryanodine receptor involved in coupling with L-type Ca2+ channels. Nakai J, Sekiguchi N, Rando TA, Allen PD, Beam KG. J Biol Chem. 1998 May 29;273(22):13403-6. PubMed 9593671
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