pCIG3_mCherry_Rab5_DN
(Plasmid
#75111)
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Purposeexpression of mCherry-Rab5 DN and EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75111 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIG3
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRAB5A
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SpeciesH. sapiens (human)
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MutationDN S34N
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Entrez GeneRAB5A (a.k.a. RAB5)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer mCherry-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG3_mCherry_Rab5_DN was a gift from Felicia Goodrum (Addgene plasmid # 75111 ; http://n2t.net/addgene:75111 ; RRID:Addgene_75111)