pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
(Plasmid
#75082)
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PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOTTC374 - pAAV EF1a DIO iRFP
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Backbone manufacturerChristopher Richie / OTTC / NIDA
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6442
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNuc-eYFP
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Alt nameNuclear-localized enhanced yellow fluorescent protein
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Insert Size (bp)813
- Promoter EF1a
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer EF1a_F1
- 3′ sequencing primer WPRE_R1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP was a gift from Brandon Harvey (Addgene plasmid # 75082 ; http://n2t.net/addgene:75082 ; RRID:Addgene_75082) -
For your References section:
Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions that Are Relayed to the Ventral Tegmental Area to Regulate Learning. Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G. Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 10.1016/j.cub.2017.06.024. Epub 2017 Jul 6. 10.1016/j.cub.2017.06.024 PubMed 28690111