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pOTTC710 - pAAV EF1a DIO Mem-AcGFP
(Plasmid #75081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC374 - pAAV EF1a DIO iRFP
  • Backbone manufacturer
    Christopher Richie / OTTC / NIDA
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6418
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mem-AcGFP
  • Alt name
    Membrane-localized AcGFP
  • Insert Size (bp)
    774
  • Promoter EF1a
  • Tag / Fusion Protein
    • Palmitylation site (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a_F1
  • 3′ sequencing primer WPRE_R1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC710 - pAAV EF1a DIO Mem-AcGFP was a gift from Brandon Harvey (Addgene plasmid # 75081 ; http://n2t.net/addgene:75081 ; RRID:Addgene_75081)

  • For your References section:

    Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions that Are Relayed to the Ventral Tegmental Area to Regulate Learning. Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G. Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 10.1016/j.cub.2017.06.024. Epub 2017 Jul 6. 10.1016/j.cub.2017.06.024 PubMed 28690111
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