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Addgene

pJK021
(Plasmid #75060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC118
  • Backbone size w/o insert (bp) 3162
  • Total vector size (bp) 4842
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphoribosylaminoimidazole carboxylase ADE2
  • Alt name
    N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein
  • Alt name
    YOR128C
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    E. coli optimized codons in first 25 residues
  • GenBank ID
    NP_014771
  • Entrez Gene
    ADE2 (a.k.a. YOR128C)
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).

In JM109 cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK021 was a gift from T. J. Kappock & JoAnne Stubbe (Addgene plasmid # 75060 ; http://n2t.net/addgene:75060 ; RRID:Addgene_75060)
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