pJJR82
(Plasmid #75027)

• Purpose: EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms
• Depositing Lab: Mike Boxem
• Publication: Ramalho et al Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.

Backbone

• Vector backbone: pDD282
• Backbone size w/o insert (bp): 3854
• Total vector size (bp): 10481
• Modifications to backbone: Replaced GFP with EGFP
• Vector type: Worm Expression, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. The midiprep yield from this vector is generally low. The depositing lab uses the low-copy procedure of the plasmid isolation kit.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP^SEC^3xFlag
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 6627
• Tags / Fusion Proteins:
  • C. elegans codon optimized EGFP
  • 3xFLAG

Resource Information

• Supplemental Documents:
  • Annotated ApE file
• A portion of this plasmid was derived from a plasmid made by: Synthetic construct.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry