p5E-gfap
(Plasmid #75024)

• Purpose: Can be used to drive expression in zebrafish astrocytes.
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pDONRP2R-P3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2763
• Total vector size (bp): 10181
• Modifications to backbone: n/a
• Vector type: 5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: glial fibrillary acidic protein promoter
• Alt name: gfap
• Species: D. rerio (zebrafish)
• Insert Size (bp): 7398
• Entrez Gene: gfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
• Promoter: gfap

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This promoter was subcloned from peGFP-gfap (Bernardos et al., 2006, Addgene #39761)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry