p5E-mpeg1.1
(Plasmid #75023)

• Purpose: Can be used to drive expression in zebrafish microglia.
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pDONRP2R-P3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2763
• Total vector size (bp): 4619
• Modifications to backbone: n/a
• Vector type: 5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: macrophage expressed 1, tandem duplicate 1
• Alt name: mpeg1, mpeg1.1
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1854
• Entrez Gene: mpeg1.1 (a.k.a. fj09e08, mpeg, mpeg1, wu:fb14d06, wu:fj09e08, zgc:66409)
• Promoter: mpeg1.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This promoter was subcloned from pTol2-mpeg-dendra plasmid (Harvie et al., 2013, Addgene #51462)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry