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Addgene

MIG-Luciferase-IRES-GFP
(Plasmid #75021)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 7880
  • Vector type
    Retroviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Alt name
    Luc2
  • Insert Size (bp)
    1650

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAC ATC GTG ACC TGG GAA GCC TTG G
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIG-Luciferase-IRES-GFP was a gift from Saradhi Mallampati (Addgene plasmid # 75021 ; http://n2t.net/addgene:75021 ; RRID:Addgene_75021)
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