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MI-Luciferase-IRES-mCherry
(Plasmid #75020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7880
  • Vector type
    Retroviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    firefly Luciferase
  • Alt name
    Luc2
  • Insert Size (bp)
    1653

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAC ATC GTG ACC TGG GAA GCC TTG G
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    720

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer TAC ATC GTG ACC TGG GAA GCC TTG G
  • 3′ sequencing primer CTT GGT CAC CTT CAG CTT GGC GGT C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MI-Luciferase-IRES-mCherry was a gift from Xiaoping Sun (Addgene plasmid # 75020 ; http://n2t.net/addgene:75020 ; RRID:Addgene_75020)

  • For your References section:

    Integrated genetic approaches identify the molecular mechanisms of Sox4 in early B-cell development: intricate roles for RAG1/2 and CK1epsilon. Mallampati S, Sun B, Lu Y, Ma H, Gong Y, Wang D, Lee JS, Lin K, Sun X. Blood. 2014 Jun 26;123(26):4064-76. doi: 10.1182/blood-2013-12-543801. Epub 2014 Apr 30. 10.1182/blood-2013-12-543801 PubMed 24786772
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