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Addgene

MI-Luciferase
(Plasmid #75018)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7150
  • Modifications to backbone
    GFP is excised from MIGR1 vector
  • Vector type
    Retroviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    firefly Luciferase
  • Alt name
    Luc2
  • Insert Size (bp)
    1653

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAC ATC GTG ACC TGG GAA GCC TTG G
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MI-Luciferase was a gift from Xiaoping Sun (Addgene plasmid # 75018 ; http://n2t.net/addgene:75018 ; RRID:Addgene_75018)

  • For your References section:

    Tyrosine kinase inhibitors induce mesenchymal stem cell-mediated resistance in BCR-ABL+ acute lymphoblastic leukemia. Mallampati S, Leng X, Ma H, Zeng J, Li J, Wang H, Lin K, Lu Y, Yang Y, Sun B, Gong Y, Lee JS, Konopleva M, Andreeff M, Arlinghaus RB, Cai Z, Fang B, Shen H, Han X, Hirsch-Ginsberg CF, Gao X, Paranjape AN, Mani SA, Clise-Dwyer K, Sun X. Blood. 2015 May 7;125(19):2968-73. doi: 10.1182/blood-2014-05-576421. Epub 2015 Feb 23. 10.1182/blood-2014-05-576421 PubMed 25712988
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