PXPR007 sgGFP-1
(Plasmid
#74960)
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PurposeCas9 + sgGFP-1 with blasticidin selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXPR_BRD001
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Backbone manufacturerBroad Institute - Genetic Perturbation Platform
- Backbone size w/o insert (bp) 13246
- Total vector size (bp) 11385
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Modifications to backbonePuromycin resistance replaced with blasticidin resistance
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Vector typeMammalian Expression, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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gRNA/shRNA sequenceGGCGAGGGCGATGCCACCTA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bsmb1 (unknown if destroyed)
- 3′ cloning site Bsmb1 (unknown if destroyed)
- 5′ sequencing primer GTATGTCTGTTGCTATTATGTCTACTATTCTTTCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PXPR007 sgGFP-1 was a gift from William Hahn (Addgene plasmid # 74960 ; http://n2t.net/addgene:74960 ; RRID:Addgene_74960) -
For your References section:
ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to MAPK Pathway Inhibition. Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE, Hahn WC. Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 10.1016/j.celrep.2017.01.031. 10.1016/j.celrep.2017.01.031 PubMed 28178529