pBABE-puroGFP-Eps8WT
(Plasmid
#74956)
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Purposemammalian expression of Eps8 fused to GFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 8300
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEps8
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SpeciesM. musculus (mouse)
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Insert Size (bp)3100
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GenBank ID13860
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Entrez GeneEps8
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBABE5'
- 3′ sequencing primer pBABE3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-puroGFP-Eps8WT was a gift from Giorgio Scita (Addgene plasmid # 74956 ; http://n2t.net/addgene:74956 ; RRID:Addgene_74956) -
For your References section:
The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration. Frittoli E, Matteoli G, Palamidessi A, Mazzini E, Maddaluno L, Disanza A, Yang C, Svitkina T, Rescigno M, Scita G. Immunity. 2011 Sep 23;35(3):388-99. doi: 10.1016/j.immuni.2011.07.007. Epub 2011 Aug 11. 10.1016/j.immuni.2011.07.007 PubMed 21835647