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Addgene

pBABE-puroGFP-Eps8WT
(Plasmid #74956)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74956 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 8300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Eps8
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3100
  • GenBank ID
    13860
  • Entrez Gene
    Eps8
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer pBABE3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puroGFP-Eps8WT was a gift from Giorgio Scita (Addgene plasmid # 74956 ; http://n2t.net/addgene:74956 ; RRID:Addgene_74956)
  • For your References section:

    The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration. Frittoli E, Matteoli G, Palamidessi A, Mazzini E, Maddaluno L, Disanza A, Yang C, Svitkina T, Rescigno M, Scita G. Immunity. 2011 Sep 23;35(3):388-99. doi: 10.1016/j.immuni.2011.07.007. Epub 2011 Aug 11. 10.1016/j.immuni.2011.07.007 PubMed 21835647