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pCIG1_UL136_23kDa_Myc
(Plasmid #74949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIG1
  • Backbone size w/o insert (bp) 7535
  • Total vector size (bp) 7900
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UL136
  • Alt name
    HCMV UL136 protein, subcloned from TB40E
  • Species
    Human Cytomegalovirus strain TB40/E
  • Insert Size (bp)
    453
  • Mutation
    delta 1-99 M128A
  • GenBank ID
    EF999921.1 ABV71655.1
  • Entrez Gene
    UL136 (a.k.a. HHV5wtgp125)
  • Promoter CMV, Lentiviral LTR
  • Tag / Fusion Protein
    • HA Tag (YPYDVPDYA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned by Dr. Katie Caviness. Expresses ONLY the 23kda isoform, expression of all other isoforms has been suppressed by mutating initiating methionine residues to alanine. Amino acids 1-99 are not present in this clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG1_UL136_23kDa_Myc was a gift from Felicia Goodrum (Addgene plasmid # 74949 ; http://n2t.net/addgene:74949 ; RRID:Addgene_74949)

  • For your References section:

    Complex expression of the UL136 gene of human cytomegalovirus results in multiple protein isoforms with unique roles in replication. Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F. J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 2014 Oct 8. 10.1128/JVI.02711-14 PubMed 25297993
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