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pGAMA-YAP
(Plasmid #74942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAMA-empty
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YAP
  • Alt name
    YAP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter EF1α
  • Tag / Fusion Protein
    • T2A-mCherry

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YAP introduced by Gateway reaction (with stop codon removed) will be driven by EF1a promoter, with a V5 C-terminal tag followed by T2A mCherry. Cells will turn red fluorescent.

Qin et al. YAP Induces Human Naive Pluripotency. 2016 Mar 15;14(10):2301-12.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAMA-YAP was a gift from Miguel Ramalho-Santos (Addgene plasmid # 74942 ; http://n2t.net/addgene:74942 ; RRID:Addgene_74942)

  • For your References section:

    YAP Induces Human Naive Pluripotency. Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos M. Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3. 10.1016/j.celrep.2016.02.036 PubMed 26947063
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