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pcDNA3-Flag-NSF/T645A
(Plasmid #74936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-NTAP
  • Backbone manufacturer
    LifeTechnologies
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NSF
  • Alt name
    N-ethylmaleimide sensitive fusion
  • Species
    H. sapiens (human)
  • Mutation
    T645A (mutation in the D2 domain of the protein)
  • Entrez Gene
    NSF (a.k.a. DEE96, SEC18, SKD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-NSF/T645A was a gift from Elisa Greggio (Addgene plasmid # 74936 ; http://n2t.net/addgene:74936 ; RRID:Addgene_74936)

  • For your References section:

    LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate. Belluzzi E, Gonnelli A, Cirnaru MD, Marte A, Plotegher N, Russo I, Civiero L, Cogo S, Carrion MP, Franchin C, Arrigoni G, Beltramini M, Bubacco L, Onofri F, Piccoli G, Greggio E. Mol Neurodegener. 2016 Jan 13;11(1):1. doi: 10.1186/s13024-015-0066-z. 10.1186/s13024-015-0066-z [pii] PubMed 26758690
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