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Addgene

pRRLsin.PPT.CMV­‐GFP­‐Eps8 WT
(Plasmid #74922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLsin.PPT.CMV
  • Backbone manufacturer
    L. Naldini, San Raffaele, Milano
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Eps8
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3100
  • GenBank ID
    13860
  • Entrez Gene
    Eps8
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLsin.PPT.CMV­‐GFP­‐Eps8 WT was a gift from Giorgio Scita (Addgene plasmid # 74922 ; http://n2t.net/addgene:74922 ; RRID:Addgene_74922)

  • For your References section:

    Eps8 regulates axonal filopodia in hippocampal neurons in response to brain-derived neurotrophic factor (BDNF). Menna E, Disanza A, Cagnoli C, Schenk U, Gelsomino G, Frittoli E, Hertzog M, Offenhauser N, Sawallisch C, Kreienkamp HJ, Gertler FB, Di Fiore PP, Scita G, Matteoli M. PLoS Biol. 2009 Jun 30;7(6):e1000138. doi: 10.1371/journal.pbio.1000138. Epub 2009 Jun 30. 10.1371/journal.pbio.1000138 PubMed 19564905
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