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pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
(Plasmid #74919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-CAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Klf2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Klf2 (a.k.a. Lklf)
  • Promoter CAG
  • Tag / Fusion Protein
    • T2A (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Nanog
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo was a gift from Jose Silva (Addgene plasmid # 74919 ; http://n2t.net/addgene:74919 ; RRID:Addgene_74919)

  • For your References section:

    MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, Hendrich B, Silva JC. Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15. 10.1016/j.stem.2014.04.019 PubMed 24835571
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