pPB-CAG-rNanog-pgk-hph
(Plasmid
#74917)
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PurposeMammalian expression of rNanog
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPB-CAG
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNanog
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SpeciesR. norvegicus (rat)
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Entrez GeneNanog
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-rNanog-pgk-hph was a gift from Jose Silva (Addgene plasmid # 74917 ; http://n2t.net/addgene:74917 ; RRID:Addgene_74917) -
For your References section:
Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, Pain B, Castro LF, Silva JC. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775. 10.1242/dev.068775 PubMed 22028025
