pPB-CAG-Nanog-pA-pgk-hph
(Plasmid
#74915)
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PurposeMammalian expression of mNanog
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPB-CAG
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNanog
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SpeciesM. musculus (mouse)
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Entrez GeneNanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-Nanog-pA-pgk-hph was a gift from Jose Silva (Addgene plasmid # 74915 ; http://n2t.net/addgene:74915 ; RRID:Addgene_74915) -
For your References section:
Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions. Theunissen TW, van Oosten AL, Castelo-Branco G, Hall J, Smith A, Silva JC. Curr Biol. 2011 Jan 11;21(1):65-71. doi: 10.1016/j.cub.2010.11.074. Epub 2010 Dec 30. 10.1016/j.cub.2010.11.074 PubMed 21194951