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Addgene

pPB-CAG-Flag3-pG-mNanog-pgk-hph
(Plasmid #74911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-CAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nanog
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-CAG-Flag3-pG-mNanog-pgk-hph was a gift from Jose Silva (Addgene plasmid # 74911 ; http://n2t.net/addgene:74911 ; RRID:Addgene_74911)

  • For your References section:

    Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, Pain B, Castro LF, Silva JC. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775. 10.1242/dev.068775 PubMed 22028025
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