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Addgene

pEBB HA CCDC22
(Plasmid #74903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CCDC22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1883
  • GenBank ID
    NM_014008
  • Entrez Gene
    CCDC22 (a.k.a. CXorf37, JM1, RTSC2)
  • Promoter EF-1alpha promoter
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTC AAG CCT CAG ACA GTG G
  • 3′ sequencing primer GAG GGG TGC AAG GAC CCA G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB HA CCDC22 was a gift from Ezra Burstein (Addgene plasmid # 74903 ; http://n2t.net/addgene:74903 ; RRID:Addgene_74903)

  • For your References section:

    CCDC22 deficiency in humans blunts activation of proinflammatory NF-kappaB signaling. Starokadomskyy P, Gluck N, Li H, Chen B, Wallis M, Maine GN, Mao X, Zaidi IW, Hein MY, McDonald FJ, Lenzner S, Zecha A, Ropers HH, Kuss AW, McGaughran J, Gecz J, Burstein E. J Clin Invest. 2013 May;123(5):2244-56. doi: 10.1172/JCI66466. Epub 2013 Apr 8. 10.1172/JCI66466 PubMed 23563313
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