pEBB HA RelA
(Plasmid #74892)

• Purpose: Express RelA in mammalian cells
• Depositing Labs: Ezra Burstein, Colin Duckett
• Publication: Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30.

Backbone

• Vector backbone: pEBB
• Backbone size w/o insert (bp): 5350
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RelA
• Species: H. sapiens (human)
• Insert Size (bp): 1656
• GenBank ID: NM_021975
• Entrez Gene: RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
• Promoter: EF-1alpha promoter
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CTC AAG CCT CAG ACA GTG G
• 3′ sequencing primer: GAG GGG TGC AAG GAC CCA G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry