pEGFP C1-Eps8 ∆cap/∆bund
(Plasmid
#74891)
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Purposemammalian expression of the capping and bundling activity mutant Eps8 fused to GFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 7100
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEps8
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SpeciesM. musculus (mouse)
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Insert Size (bp)2400
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MutationV689D, L693D, L757A, K759A
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GenBank ID13860
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Entrez GeneEps8
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP C1-Eps8 ∆cap/∆bund was a gift from Giorgio Scita (Addgene plasmid # 74891 ; http://n2t.net/addgene:74891 ; RRID:Addgene_74891) -
For your References section:
Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping. Hertzog M, Milanesi F, Hazelwood L, Disanza A, Liu H, Perlade E, Malabarba MG, Pasqualato S, Maiolica A, Confalonieri S, Le Clainche C, Offenhauser N, Block J, Rottner K, Di Fiore PP, Carlier MF, Volkmann N, Hanein D, Scita G. PLoS Biol. 2010 Jun 1;8(6):e1000387. doi: 10.1371/journal.pbio.1000387. 10.1371/journal.pbio.1000387 PubMed 20532239