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Addgene

pGWB529
(Plasmid #74871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPZP221
  • Backbone manufacturer
    Hajdukiewicz P., Svab Z. and Maliga P.
  • Backbone size (bp) 8500
  • Modifications to backbone
    gentamycin-resistant marker was removed and hygromycin-resistant marker (NOS promoter:HPT:NOS terminator) was inserted
  • Vector type
    Plant Expression
  • Promoter CaMV35S
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB529 was a gift from Tsuyoshi Nakagawa (Addgene plasmid # 74871 ; http://n2t.net/addgene:74871 ; RRID:Addgene_74871)

  • For your References section:

    Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S. Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7. 10.1271/bbb.70216 PubMed 17690442
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