pGWB501
(Plasmid #74843)

• Purpose: (Empty Backbone) Gateway cloning compatible binary vector for simple cloning of gene.
• Depositing Lab: Tsuyoshi Nakagawa
• Publication: Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.

Backbone

• Vector backbone: pPZP221
• Backbone manufacturer: Hajdukiewicz P., Svab Z. and Maliga P.
• Backbone size (bp): 8500
• Modifications to backbone: gentamycin-resistant marker was removed and hygromycin-resistant marker (NOS promoter:HPT:NOS terminator) was inserted
• Vector type: Plant Expression
• Promoter: No Promoter
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that pUC primers are not recommended for sequencing due to the inverted repeat of the Tnos sequence which forms a secondary structure and prevents sequencing reactions. If you need to sequence or PCR the Tnos region, digestion by AscI helps separates two Tnos sequences and prevents the secondary structure.