pGWB427
(Plasmid
#74821)
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Purpose(Empty Backbone) Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPZP221
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Backbone manufacturerHajdukiewicz P., Svab Z. and Maliga P.
- Backbone size (bp) 8500
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Modifications to backbonegentamycin-resistant marker was removed and kanamycin-resistant marker (NOS promoter:NPTII:NOS terminator) was inserted.
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Vector typePlant Expression
- Promoter CaMV35S
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB427 was a gift from Tsuyoshi Nakagawa (Addgene plasmid # 74821 ; http://n2t.net/addgene:74821 ; RRID:Addgene_74821) -
For your References section:
Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S. Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7. 10.1271/bbb.70216 PubMed 17690442
