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Addgene

pEBB Flag GCN5
(Plasmid #74784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBB
  • Backbone size w/o insert (bp) 5350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCN5
  • Alt name
    KAT2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2514
  • GenBank ID
    NM_021078
  • Entrez Gene
    KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
  • Promoter EF-1alpha promoter
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CTC AAG CCT CAG ACA GTG G
  • 3′ sequencing primer GAG GGG TGC AAG GAC CCA G
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB Flag GCN5 was a gift from Ezra Burstein (Addgene plasmid # 74784 ; http://n2t.net/addgene:74784 ; RRID:Addgene_74784)

  • For your References section:

    GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, Burstein E. Genes Dev. 2009 Apr 1;23(7):849-61. doi: 10.1101/gad.1748409. 10.1101/gad.1748409 PubMed 19339690
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