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pET21a-Chaetomium thermophilum LIC
(Plasmid #74782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5385
  • Total vector size (bp) 7029
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 DE3 strain for protein production
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chaetomium thermophilum dynein light intermediate chain
  • Alt name
    LIC
  • Species
    Chaetomium thermophilum
  • Insert Size (bp)
    1644
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-Chaetomium thermophilum LIC was a gift from Ron Vale (Addgene plasmid # 74782 ; http://n2t.net/addgene:74782 ; RRID:Addgene_74782)

  • For your References section:

    A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. Schroeder CM, Ostrem JM, Hertz NT, Vale RD. Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351. 10.7554/eLife.03351 PubMed 25272277
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