pGEX6P1-C. thermophilum LIC
(Plasmid
#74780)
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Purposeencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74780 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX6P1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4981
- Total vector size (bp) 6625
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsis in BL21 DE3 strain, used for protein production
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedynein light intermediate chain (Chaetomium thermophilum)
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Alt nameLIC1
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SpeciesChaetomium thermophilum
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Insert Size (bp)1644
- Promoter T7
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P1-C. thermophilum LIC was a gift from Ron Vale (Addgene plasmid # 74780 ; http://n2t.net/addgene:74780 ; RRID:Addgene_74780) -
For your References section:
A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. Schroeder CM, Ostrem JM, Hertz NT, Vale RD. Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351. 10.7554/eLife.03351 PubMed 25272277
