MSCV-dGFP-JARID1C A388P
(Plasmid
#74778)
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Purposeretroviral expression of JARID1C A388P
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameJARID1C
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SpeciesH. sapiens (human)
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MutationA388P
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Entrez GeneKDM5C (a.k.a. DXS1272E, JARID1C, MRX13, MRXJ, MRXSCJ, MRXSJ, SMCX, XE169)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP is driven by a separate promoter (UBC promoter), and is used only to track cells and the efficiency of infection/transfection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-dGFP-JARID1C A388P was a gift from Giovanni Tonon (Addgene plasmid # 74778 ; http://n2t.net/addgene:74778 ; RRID:Addgene_74778) -
For your References section:
Histone demethylase JARID1C inactivation triggers genomic instability in sporadic renal cancer. Rondinelli B, Rosano D, Antonini E, Frenquelli M, Montanini L, Huang D, Segalla S, Yoshihara K, Amin SB, Lazarevic D, The BT, Verhaak RG, Futreal PA, Di Croce L, Chin L, Cittaro D, Tonon G. J Clin Invest. 2015 Dec;125(12):4625-37. doi: 10.1172/JCI81040. Epub 2015 Nov 9. 10.1172/JCI81040 PubMed 26551685