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pETDuet-msGC-NT2
(Plasmid #74763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 7870
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    fragment of alfa subunit of sGC
  • Alt name
    alpha msGC
  • Species
    manduca sexta (tobacco hornworm)
  • Insert Size (bp)
    1311
  • Mutation
    fragment 49-471 of the alfa subunit
  • GenBank ID
    AF062750
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BanHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ATGCGTCCGGCGTAGA
  • 3′ sequencing primer GATTATGCGGCCGTGTACAA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    fragment of beta subunit of sGC
  • Alt name
    beta msGC
  • Species
    manduca sexta (tobacco hornworm)
  • Insert Size (bp)
    1203
  • GenBank ID
    AF062751
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcorI (not destroyed)
  • 5′ sequencing primer TTGTACACGGCCGCATAATC
  • 3′ sequencing primer GCTAGTTATTGCTCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-msGC-NT2 was a gift from William Montfort (Addgene plasmid # 74763 ; http://n2t.net/addgene:74763 ; RRID:Addgene_74763)

  • For your References section:

    Allostery in recombinant soluble guanylyl cyclase from Manduca sexta. Hu X, Murata LB, Weichsel A, Brailey JL, Roberts SA, Nighorn A, Montfort WR. J Biol Chem. 2008 Jul 25;283(30):20968-77. doi: 10.1074/jbc.M801501200. Epub 2008 May 30. 10.1074/jbc.M801501200 PubMed 18515359
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