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Addgene

pGAMA-empty
(Plasmid #74755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAMA-empty
  • Backbone size (bp) 9957
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter EF1a
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway destination lentiviral vector. Gene of interest introduced by Gateway reaction (with stop codon removed) will be driven by EF1a promoter, with a V5 C-terminal tag followed by T2A mCherry. Cells will turn red fluorescent.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAMA-empty was a gift from Miguel Ramalho-Santos (Addgene plasmid # 74755 ; http://n2t.net/addgene:74755 ; RRID:Addgene_74755)

  • For your References section:

    YAP Induces Human Naive Pluripotency. Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos M. Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3. 10.1016/j.celrep.2016.02.036 PubMed 26947063
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