pET28a-FIP3
(Plasmid #74753)

• Purpose: Expressed sfGFP-tagged FIP3 in E. coli for protein production
• Depositing Lab: Ron Vale
• Publication: Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 6198
• Total vector size (bp): 8466
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3) for protein production
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab11 family interacting protein 3
• Alt name: FIP3
• Alt name: RAB11FIP3
• Species: H. sapiens (human)
• Insert Size (bp): 2268
• GenBank ID:
• Entrez Gene: RAB11FIP3 (a.k.a. CART1, FIP3-Rab11, Rab11-FIP3)
• Promoter: T7
• Tags / Fusion Proteins:
  • superfolder GFP (N terminal on insert)
  • Strep II (N terminal on insert)
  • 6x His (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry