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PurposeGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDestTol2pA2
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Vector typezebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
- Promoter nine GC-responsive elements (9xGRE) from the rat tyrosine aminotransferase promoter together with the thymidine kinase promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-N
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDestTol2pA2-9xGRE was a gift from Luisa Dalla Valle (Addgene plasmid # 74736 ; http://n2t.net/addgene:74736 ; RRID:Addgene_74736) -
For your References section:
A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish. Benato F, Colletti E, Skobo T, Moro E, Colombo L, Argenton F, Dalla Valle L. Mol Cell Endocrinol. 2014 Jul 5;392(1-2):60-72. doi: 10.1016/j.mce.2014.04.015. Epub 2014 May 4. 10.1016/j.mce.2014.04.015 PubMed 24796658
Map uploaded by the depositor.
