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Addgene

pDestTol2pA2-9xGRE
(Plasmid #74736)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDestTol2pA2
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • Promoter nine GC-responsive elements (9xGRE) from the rat tyrosine aminotransferase promoter together with the thymidine kinase promoter

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA2-9xGRE was a gift from Luisa Dalla Valle (Addgene plasmid # 74736 ; http://n2t.net/addgene:74736 ; RRID:Addgene_74736)
  • For your References section:

    A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish. Benato F, Colletti E, Skobo T, Moro E, Colombo L, Argenton F, Dalla Valle L. Mol Cell Endocrinol. 2014 Jul 5;392(1-2):60-72. doi: 10.1016/j.mce.2014.04.015. Epub 2014 May 4. 10.1016/j.mce.2014.04.015 PubMed 24796658