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Addgene

pdRBFC-VP35-YC
(Plasmid #74726)

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Item Catalog # Description Quantity Price (USD)
Plasmid 74726 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEarley-202
  • Backbone manufacturer
    The Plant Journal (2006) 45, 616–629
  • Total vector size (bp) 10757
  • Vector type
    Plant Expression ; double-stranded RNA labeling
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Marburg virus VP35 protein, partial
  • Alt name
    VP35
  • Species
    Virus
  • Insert Size (bp)
    381
  • Promoter CaMV 35S
  • Tags / Fusion Proteins
    • FLAG
    • YFP C-terminal half domain

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 35S-F: CGCAAGACCCTTCCTCTATATAAGGAA
  • 3′ sequencing primer OSC-R: ATCTCATTAAAGCAGGACTCTAGGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Erica O.Saphire (The Scripps Research Institute, CA,USA).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdRBFC-VP35-YC was a gift from Aiming Wang (Addgene plasmid # 74726)

  • For your References section:

    Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation. Cheng X, Deng P, Cui H, Wang A. Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. Epub 2015 Sep 7. 10.1016/j.virol.2015.08.023 PubMed 26351203
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