pCX-HO1-2A-EGFP
(Plasmid #74672)

• Purpose: Mammalian expression of HO1 and EGFP
• Depositing Lab: Roberto Giovannoni
• Publication: Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. doi: 10.1371/journal.pone.0141933. eCollection 2015.

Backbone

• Vector backbone: pCX-2A-2A
• Backbone manufacturer: Giovannoni lab, Addgene plasmid 74670
• Total vector size (bp): 7478
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HO1
• Species: H. sapiens (human)
• Insert Size (bp): 1674
• Entrez Gene: HMOX1 (a.k.a. HMOX1D, HO-1, HSP32, bK286B10)
• Promoter: pCAGGS
• Tag / Fusion Protein:
  • 2A-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: pCAX-F CAGCTCCTGGGCAACGTGC
• 3′ sequencing primer: M13-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HMOX1 (hHO-1) sequence was kindly provided by Prof. Fritz Bach's laboratory at Harvard Medical School, Boston.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry