pGEM-floxedEGFP-TRI2A
(Plasmid #74669)

• Purpose: floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
• Depositing Lab: Roberto Giovannoni
• Publication: Roberto Giovannoni plasmids (unpublished)

Backbone

• Vector backbone: pGEM
• Backbone manufacturer: Promega
• Total vector size (bp): 12169
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
• Alt name: SA/LoxP-EGFP-LoxP/hHO1/F2A/hCD73/F2A/hCD39
• Promoter: pCAGGS

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HMOX1 (hHO-1) sequence was kindly provided by Prof. Fritz Bach's laboratory at Harvard Medical School, Boston. NT5E (hCD73) sequence was kindly provided by Dr. Jozef Spychala at University of Michigan, Ann Arbor. ENTPD1 (hCD39) sequence was kindly provided by Dr. Simon Robson at Harvard Medical School, Boston.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more details on TRI-2A, see pCX-TRI-2A, Addgene plasmid 74674.

Contains a D473N mutation in CD73, but it does not impair either the plasmid function or the hCD73 protein activity.