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Addgene

Mmadhc
(Plasmid #74662)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mmadhc
  • Alt name
    MMADHCMMA-c001
  • Alt name
    MMADHCMMAmethylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    601
  • Entrez Gene
    MMADHC (a.k.a. C2orf25, CL25022, cblD)
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|19527054

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mmadhc was a gift from Nicola Burgess-Brown (Addgene plasmid # 74662 ; http://n2t.net/addgene:74662 ; RRID:Addgene_74662)
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