ACVR1A
(Plasmid
#74650)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFB-LIC-Bse
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACVR1A
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Alt nameACVR1A-c097
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Alt nameACVR1activin A receptor, type I
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SpeciesH. sapiens (human)
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Insert Size (bp)1021
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MutationR206H
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Entrez GeneACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
- Promoter PH
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Tag
/ Fusion Protein
- His-6-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. gi|4501895
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACVR1A was a gift from Nicola Burgess-Brown (Addgene plasmid # 74650 ; http://n2t.net/addgene:74650 ; RRID:Addgene_74650)