JARID1BA
(Plasmid
#74648)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFB-LIC-Bse
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJARID1BA
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Alt nameJARID1BA-c517
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Alt nameKDM5BJumonji, AT rich interactive domain 1B (RBP2-like); putative DNA/chromatin binding motif_
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SpeciesH. sapiens (human)
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Insert Size (bp)1553
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MutationLeu101-(Gly)4-Ala374 deletion mutant
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Entrez GeneKDM5B (a.k.a. CT31, JARID1B, MRT65, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1)
- Promoter PH
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Tag
/ Fusion Protein
- His-6-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. gi|19923370
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JARID1BA was a gift from Nicola Burgess-Brown (Addgene plasmid # 74648 ; http://n2t.net/addgene:74648 ; RRID:Addgene_74648)