Skip to main content
Addgene

TIF1A
(Plasmid #74647)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TIF1A
  • Alt name
    TIF1A-c042
  • Alt name
    TRIM24transcriptional intermediary factor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    888
  • Entrez Gene
    TRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7/lac regulated, N-terminal His-tag, SUMO, restriction cloned with NdeI/XhoI gi|14971415

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TIF1A was a gift from Nicola Burgess-Brown (Addgene plasmid # 74647 ; http://n2t.net/addgene:74647 ; RRID:Addgene_74647)
Related items:
Commonly requested with: