POLQA
(Plasmid #74645)

• Purpose: Baculovirus expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC 2016 plasmids (unpublished)

Backbone

• Vector backbone: pFB-LIC-Bse
• Vector type: Baculovirus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POLQA
• Alt name: POLQA-c232
• Alt name: POLQDNA polymerase theta [isoform 1]
• Species: H. sapiens (human)
• Insert Size (bp): 2559
• Entrez Gene: POLQ (a.k.a. PRO0327)
• Promoter: PH
• Tag / Fusion Protein:
  • His-6-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: Fbac-1
• 3′ sequencing primer: Fbac-2

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. gi|40805845