pIM23
(Plasmid #74643)

• Purpose: Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker
• Depositing Lab: Jiří Hašek
• Publication: Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027.

Backbone

• Vector backbone: pUG23
• Backbone manufacturer: U. Gueldener
• Backbone size w/o insert (bp): 6303
• Total vector size (bp): 6317
• Modifications to backbone: Replacement of yEGFP with yTagRFP-T
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: yTagRFP-T
• Alt name: fluorescent protein
• Promoter: MET25

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13reverse
• 3′ sequencing primer: M13fw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Whiteway, McGill University, Montreal, Canada

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry