pIM701
(Plasmid #74642)

• Purpose: alias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection marker
• Depositing Lab: Jiří Hašek
• Publication: Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027.

Backbone

• Vector backbone: pFM699
• Backbone manufacturer: Marian Farkasovsky
• Backbone size w/o insert (bp): 4522
• Total vector size (bp): 4677
• Modifications to backbone: Replacement of mCherry with yTagRFP-T
• Vector type: Yeast Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ymTagBFP

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Kaern, University Ottawa, Canada

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry