pFM699
(Plasmid #74636)

• Purpose: alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection marker
• Depositing Lab: Jiří Hašek
• Publication: Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027.

Backbone

• Vector backbone: pFA6a-natNT2
• Backbone manufacturer: Janke et al., (2004) Yeast 21:947-962
• Backbone size w/o insert (bp): 3784
• Total vector size (bp): 4677
• Modifications to backbone: Insertion of mCherry
• Vector type: Yeast Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Tsien, San Diego, USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry