pFM689
(Plasmid #74635)

• Purpose: alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker
• Depositing Lab: Jiří Hašek
• Publication: Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027.

Backbone

• Vector backbone: pFA6a-hphNT1
• Backbone manufacturer: Janke et al., (2004) Yeast 21:947-962
• Backbone size w/o insert (bp): 4167
• Total vector size (bp): 4677
• Modifications to backbone: Insertion of mCherry
• Vector type: Yeast Expression
• Promoter: none
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Tsien, San Diego, USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to the published sequence of the pFA6a-hphNT1 backbone vector (Janke, et al., 2004, Yeast 21: 947-962) we found one PstI site missing in its sequence. It is the site in the vector –promoter part and there is a substitution of A by G in position 284 bp that arose most probably during construction of preceding cassettes, e.g. pFA6-hphMX4. We introduced the substitution also into maps of our modules carrying the hygromycin resistance - pFM689, pIM690 and pIM691.