p5E--3mnx1
(Plasmid #74632)

• Purpose: Can be used to drive expression specifically in zebrafish motor neurons
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pDONRP2R-P3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2642
• Total vector size (bp): 5507
• Modifications to backbone: n/a
• Vector type: 5' entry vector for Multisite Gateway Three Fragment vector construction kit(Invitrogen)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: motor neuron and pancreas homeobox 1 promoter
• Alt name: -3mnx1 promoter, Hb9 promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2864
• Entrez Gene: mnx1 (a.k.a. hlxb, hlxb9, zgc:112174)
• Promoter: -3mnx1

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note Addgene's quality control sequence found an 8bp gap when compared to the depositor's sequence. This gap is located in a T-rich region and does not affect plasmid function.