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pET28a-Hook3 aa 1-160-GCN4
(Plasmid #74608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 6297
  • Total vector size (bp) 6777
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3) for protein production
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human Hook3 amino acids 1-160
  • Alt name
    HOOK3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    480
  • GenBank ID
    NM_032410
  • Entrez Gene
    HOOK3 (a.k.a. HK3)
  • Tags / Fusion Proteins
    • superfolder GFP (N terminal on insert)
    • Strep II (N terminal on insert)
    • 6x His (N terminal on insert)
    • GCN4 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Hook3 aa 1-160-GCN4 was a gift from Ron Vale (Addgene plasmid # 74608 ; http://n2t.net/addgene:74608 ; RRID:Addgene_74608)

  • For your References section:

    Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3. Schroeder CM, Vale RD. J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002. 10.1083/jcb.201604002 PubMed 27482052
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